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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
17/06/2010 |
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Data da última atualização: |
04/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BENATTI, T. R.; VALICENTE, F. H.; AGGARWAL, R.; ZHAO, C.; WALLING, J. G.; CHEN, M.-S.; CAMBRON, S. E.; SCHEMERHORN, B. J.; STUART, J. J. |
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Afiliação: |
THIAGO R. BENATTI, PURDUE UNIVERSITY; FERNANDO HERCOS VALICENTE, CNPMS; RAJAT AGGARWAL, PURDUE UNIVERSITY; CHAOYANG ZHAO, PURDUE UNIVERSITY; JASON G. WALLING, PURDUE UNIVERSITY; MING-SHUN CHEN, KANSAS STATE UNIVERSITY; SUE E. CAMBRON, KANSAS STATE UNIVERSITY; BRANDON J. SCHEMERHORN, KANSAS STATE UNIVERSITY; JEFFREY J. STUART, PURDUE UNIVERSITY. |
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Título: |
A neo-sex chromosome that drives postzygotic sex determination in the Hessian fly (Mayetiola destructor). |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genetics, Austin, v. 184, n. 3, p.769-777, 2010. |
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DOI: |
10.1534/genetics.109.10.8589 |
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Idioma: |
Inglês |
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Conteúdo: |
Two nonoverlapping autosomal inversions defined unusual neo-sex chromosomes in the Hessian fly (Mayetiola destructor). Like other neo-sex chromosomes, these were normally heterozygous, present only in one sex, and suppressed recombination around a sex-determining master switch. Their unusual properties originated from the anomalous Hessian fly sex determination system in which postzygotic chromosome elimination is used to establish the sex-determining karyotypes. This system permitted the evolution of a master switch (Chromosome maintenance, Cm) that acts maternally. All of the offspring of females that carry Cm-associated neo-sex chromosomes attain a female-determining somatic karyotype and develop as females. Thus, the chromosomes act as maternal effect neo-W's, or W-prime (W') chromosomes, where ZW' females mate with ZZ males to engender female-producing (ZW') and male-producing (ZZ) females in equal numbers. Genetic mapping and physical mapping identified the inversions. Their distribution was determined in nine populations. Experimental matings established the association of the inversions with Cm and measured their recombination suppression. The inversions are the functional equivalent of the sciarid X-prime chromosomes. We speculate that W' chromosomes exist in a variety of species that produce unisexual broods. |
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Thesaurus Nal: |
Mayetiola destructor. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02070naa a2200241 a 4500
001 1855385
005 2018-06-04
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1534/genetics.109.10.8589$2DOI
100 1 $aBENATTI, T. R.
245 $aA neo-sex chromosome that drives postzygotic sex determination in the Hessian fly (Mayetiola destructor).$h[electronic resource]
260 $c2010
520 $aTwo nonoverlapping autosomal inversions defined unusual neo-sex chromosomes in the Hessian fly (Mayetiola destructor). Like other neo-sex chromosomes, these were normally heterozygous, present only in one sex, and suppressed recombination around a sex-determining master switch. Their unusual properties originated from the anomalous Hessian fly sex determination system in which postzygotic chromosome elimination is used to establish the sex-determining karyotypes. This system permitted the evolution of a master switch (Chromosome maintenance, Cm) that acts maternally. All of the offspring of females that carry Cm-associated neo-sex chromosomes attain a female-determining somatic karyotype and develop as females. Thus, the chromosomes act as maternal effect neo-W's, or W-prime (W') chromosomes, where ZW' females mate with ZZ males to engender female-producing (ZW') and male-producing (ZZ) females in equal numbers. Genetic mapping and physical mapping identified the inversions. Their distribution was determined in nine populations. Experimental matings established the association of the inversions with Cm and measured their recombination suppression. The inversions are the functional equivalent of the sciarid X-prime chromosomes. We speculate that W' chromosomes exist in a variety of species that produce unisexual broods.
650 $aMayetiola destructor
700 1 $aVALICENTE, F. H.
700 1 $aAGGARWAL, R.
700 1 $aZHAO, C.
700 1 $aWALLING, J. G.
700 1 $aCHEN, M.-S.
700 1 $aCAMBRON, S. E.
700 1 $aSCHEMERHORN, B. J.
700 1 $aSTUART, J. J.
773 $tGenetics, Austin$gv. 184, n. 3, p.769-777, 2010.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
17/07/2012 |
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Data da última atualização: |
07/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MONTE, L. G.; CONCEIÇÃO, F. R.; COUTINHO, M. L.; SEIXAS, F. K.; SILVA, E. V. da; VASCONCELLOS, F. A.; CASTRO, L. A. S. de; HARTLEBEN, C. P.; DELLAGOSTIN, O. A.; ALEIXO, J. A. G. |
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Afiliação: |
LEONARDO G. MONTE, UFPel; FABRICIO, R. CONCEIÇÃO, UFPel; MARIANA L. COUTINHO, UFPel; FABIANA K. SEIXAS, UFPel; EVERTON F. da SILVA, UFPel; FLÁVIA A. VASCONCELLOS, UFPel; LUIS ANTONIO SUITA DE CASTRO, CPACT; CLÁUDIA P. HARTLEBEN, UFPel; ODIR A. DELLAGOSTIN, UFPel; JOSÉ A. G. ALEIXO, UFPel. |
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Título: |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Comparative Immunology, Microbiology and Infectious Diseases, v. 34, n. 5, p. 441-446, set. 2011. |
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ISSN: |
0147-9571 |
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DOI: |
https://doi.org/10.1016/j.cimid.2011.08.003 |
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Idioma: |
Inglês |
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Conteúdo: |
Leptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis. |
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Palavras-Chave: |
Monoclonal. |
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Thesagro: |
Anticorpo Monoclonal; Leptospirose. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212910/1/12-Artigo-LeonardoMonte-Medicina-Veterinaria-A1.pdf
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Marc: |
LEADER 02283naa a2200289 a 4500
001 1928502
005 2021-10-07
008 2011 bl uuuu u00u1 u #d
022 $a0147-9571
024 7 $ahttps://doi.org/10.1016/j.cimid.2011.08.003$2DOI
100 1 $aMONTE, L. G.
245 $aMonoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions.$h[electronic resource]
260 $c2011
520 $aLeptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis.
650 $aAnticorpo Monoclonal
650 $aLeptospirose
653 $aMonoclonal
700 1 $aCONCEIÇÃO, F. R.
700 1 $aCOUTINHO, M. L.
700 1 $aSEIXAS, F. K.
700 1 $aSILVA, E. V. da
700 1 $aVASCONCELLOS, F. A.
700 1 $aCASTRO, L. A. S. de
700 1 $aHARTLEBEN, C. P.
700 1 $aDELLAGOSTIN, O. A.
700 1 $aALEIXO, J. A. G.
773 $tComparative Immunology, Microbiology and Infectious Diseases$gv. 34, n. 5, p. 441-446, set. 2011.
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Embrapa Clima Temperado (CPACT) |
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